Metrohm 930 Compact IC Flex Oven/SeS/Deg User Manual download pdf (Page 58)

3.20 Connecting the separation column

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930 Compact IC Flex Oven/SeS/Deg (2.930.2460)

Connecting the separation column

Removing the stoppers

CAUTION

When inserting the column, ensure that it is inserted correctly

based on the marked flow direction.

￭ Remove the stoppers from the separation column.

Installing the input of the separation column

There are 3 possibilities:

￭ Attaching the bottom end of the separation column directly to

the guard column.

￭ If the guard column is connected to the separation column using

a provided connection capillary, connect the bottom end of the

separation column to the guard column's output capillary using

the provided PEEK pressure screw (6.2744.070).

￭ If no guard column is used (not recommended), connect the col-

umn input capillary to the input of the separation column using a

short pressure screw (6.2744.070).

Rinsing the separation column

￭ Place a beaker under the separation column's outlet.

￭ Start manual control in MagIC Net and select the high-pressure

pump: Manual ▶ Manual control ▶ Pump

– Flow: in accordance with column leaflet

– On

￭ Rinse the separation column with eluent for approximately 10

minutes.

￭ Use manual control in MagIC Net to stop the high-pressure pump

again: Off.

Removing the coupling

￭ Remove the coupling (6.2744.040) from the column output capil-

lary.

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Metrohm 930 Compact IC Flex Oven/SeS/Deg User Manual Page 58