Metrohm 850 Professional IC AnCat MCS User Manual download pdf (Page 95)

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4 Operation and maintenance

850 Professional IC – AnCat – MCS

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4.7 Inline sample preparation

To protect the separation column (see Chapter 2.22, Page 64) against for-

eign particles which can affect the separating efficiency, we recommend

that all samples undergo a microfiltration (filter 0.45 µm). The ultrafiltra-

tion cell can be used for filtration (see documentation on the IC Equip-

ment for Ultrafiltration).

Samples containing significant amounts of gas should be degassed. The

sample degasser (see Chapter 2.13, Page 40) (if available) is used for

degassing.

Matrix-loaded samples (e.g. blood, oil) should be prepared for the meas-

urement by means of dialysis (see documentation on the IC Equipment for

Dialysis).

If the concentration of the sample is too high, it should be diluted before

feeding (see documentation on the IC Equipment for Sample Dilution).

For the sample preparation methods Neutralization (replacement of e.g.

with H

) and cation exchange (replacement of e.g. heavy metals

with H

), a sample preparation module (SPM) is used.

4.8 Rinsing the sample path

Before a new sample can be measured, the sample path must be rinsed

with it so that the measuring result is not falsified by the previous sample

(Sample carry-over).

In the case of automated sample feeding, the rinsing time should be at

least 3 times the transfer time. The transfer time is the time required by

the sample to flow from the sample vessel to the end of the sample loop.

Ascertaining the transfer time

The transfer time depends on the pump capacity of the peristaltic pump,

the total capillary volume and the volume of the gas removed by the sam-

ple degasser (if used) - in other words the amount of gas in the sample.

Emptying the sample path

Pump air through the sample path (pump tubing, tubing connec-

tions, capillary in the degasser, sample loop) for several minutes until

all liquid is displaced by the air.

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Metrohm 850 Professional IC AnCat MCS User Manual Page 95