Metrohm 850 Professional IC Anion MCS LP-Gradient User Manual

850 Professional IC Anion – MCS – LP Gradient – 2.850.2210Manual8.850.8053EN

Table of figures￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭VIII￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientFigure 46 Removing valves ...

5.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭90￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient￭ Place over the valve housing and hold it tightl

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭91Mounting the pump headNoteTo prevent the pum

5.6 Inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭92￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientChanging the filterThe flow must be stopped before cha

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭935.7 Inline sample preparationTo protect the

5.8 Rinsing the sample path￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭94￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient 2Aspirating the sample and measuring timeAs

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭955.9 Sample degasser5.9.1 OperationIf you a

5.11 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭96￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientIn order to record every new chroma

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭97NoteThe high pressure pump can be used for r

5.11 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭98￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient123 45Figure 50 MSM – Components1Un

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭99￭ Check whether solution emerges at the MSM

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭11 Introduction1.1 Instrument descriptionThe instrument 8

5.11 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭100￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient5.11.3.3 Replacing parts of the M

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭101￭ Insert the new MSM rotor A (50-3) in the

5.12 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭102￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientCautionThe 6.1826.xxx pump tubings consist of PVC

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭103OrdernumberName Material InnerdiameterUse6.

5.13 Metrohm CO2 suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭104￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient 3Mounting filter screw￭ Screw the fi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭1055.14 Conductivity detector5.14.1 Maintena

5.15 Separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭106￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient5.15.2 ProtectionTo protect the separation colu

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭1075.16 Quality Management and validation wit

6.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭108￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient6 Troubleshooting6.1 Problems and th

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Troubleshooting850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭109Problem Cause RemedyEluent – Evaporation of theorgani

1.1 Instrument description￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientHigh pressure pumpThe intelligent and low puls

6.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭110￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientProblem Cause RemedyMCS – vacuum pumpd

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Troubleshooting850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭111Problem Cause Remedy￭ Replace separation column (see

6.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭112￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientProblem Cause RemedyEluent – Gas bubbl

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭1137 Technical specifications7.1 Reference co

7.5 Housing￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭114￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient7.5 HousingDimensionsWidth 365 mmHeight 642 mmDepth 380 mm

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭1157.8 High pressure pumpType ￭ Serial dual-pi

7.9 Sample degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭116￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient7.9 Sample degasserMaterial fluoropolymerResistanc

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭1177.12 Metrohm Suppressor Module (MSM)Resista

7.15 Conductivity measuring system￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭118￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient7.15 Conductivity measuring systemT

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭1197.16 Mains connectionRequired voltage 100…2

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭3Conductivity detectorThe conductivity detector continuousl

7.18 Safety specification￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭120￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient7.18 Safety specificationDesign / Test ￭ EN/

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Conformity and warranty850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭1218 Conformity and warranty8.1 Declaration of

8.2 Quality Management Principles￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭122￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientEN 61010-1 Safety requirements for el

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Conformity and warranty850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭123Software developmentSoftware development occu

8.3 Warranty (guarantee)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭124￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientFaults arising from circumstances that are not

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭1259 AccessoriesCautionSubject to change without notice.9.1

9.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭126￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientQty. Order no. Description2 6.1608.020 Glass bott

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭127Qty. Order no. Description1 6.1803.040 PTFE capillary 0.5

9.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭128￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientQty. Order no. Description2 6.1816.020 Silicone t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭129Qty. Order no. Description3 6.2023.020 Clip for SGJ 14/15

1.3 About the documentation￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient1.3 About the documentationCautionPlease rea

9.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭130￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientQty. Order no. Description2 6.2621.000 Adjustable

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭131Qty. Order no. Description1 6.2621.080 Capillary cutterFo

9.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭132￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientQty. Order no. Description1 6.2743.080 Stoppers f

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭133Qty. Order no. Description2 6.2744.040 2 x UNF 10/32 coup

9.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭134￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientQty. Order no. Description3 6.2744.210 Tubing ada

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭135Qty. Order no. Description3 6.2821.090 Aspiration filterP

9.2 Optional accessories￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭136￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientQty. Order no. Description1 8.850.8053EN Manua

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭137Order no. Description6.1826.330 Pump tubing LFL (orange/w

9.2 Optional accessories￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭138￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientOrder no. Description6.1826.390 Pump tubing LF

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭139Order no. Description6.2148.010 Remote Box MSBAn addition

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭51.4 Safety instructions1.4.1 General notes on safetyWarn

9.2 Optional accessories￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭140￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientOrder no. Description6.6059.113 MagIC Net™ 1.1

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭141IndexNumbers/Symbols6.2821.090 aspiration filter ... 806.2821.

Index￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭142￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientHousing ... 114Humidity ...

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭143Rise in pressure ... 80Rollers ...

1.4 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientProtection against electrostatic chargesWarningEl

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭7More details about the disposal of your old equipment can

2.1 Front￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient2 Overview of the instrument2.1 Front912354678101112131415161

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭93Purge valveFor deaerating the high pressure

2.2 Rear￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭10￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient2.2 Rear12221219201017157654318119121381416Figure 2 Rear 850 P

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭113Mains switchFor switching the instrument o

2.2 Rear￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭12￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientCautionWhen connecting an instrument to the MSB connector (2-6)

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭133 Installation3.1 About this chapterThe Installation ch

3.2 Initial installation￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭14￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient￭ Place the detectors in the instrument and con

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭15￭ Install pump tubings.￭ Connect aspirating capillaries f

3.3 Installation of the low pressure gradient￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭16￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient3.3 Installation of the l

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭173.4 Installation diagram Figure 3Installation diagram sh

3.4 Installation diagram￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭18￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient1113567891011121314151617181920201919212224Figu

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭193Gradient mixing coil 6.2758.0204Column inlet capillaryCo

Metrohm AGCH-9101 HerisauSwitzerlandPhone +41 71 353 85 85Fax +41 71 353 89 [email protected] Professional IC Anion – MCS – LP Grad

3.5 Setting up the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭20￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient3.5 Setting up the instrument3.5.1 Packa

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭21412 3Figure 4 Connection of capillaries with pressure scr

3.7 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭22￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientCautionFor the capillary connections between

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭23321145Figure 5 Rollers and handle1Knurled screwsFor faste

3.7 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭24￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientMounting handle as MPak holderNoteWhen extend

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭253.7.2 Positioning and connecting the detectorDetector 1D

3.7 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭26￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientNoteUp to two detectors can be positioned and

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭27￭ Connect the detector cable to the detector connection s

3.7 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭28￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient123Figure 8 Connection for the leak sensor on

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭29245613798Figure 9 Drainage tubing1Drainage tubing connect

TeachwareMetrohm AGCH-9101 [email protected] documentation is protected by copyright. All rights reserved.Although all the information

3.8 Capillary and cable feed-throughs￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭30￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient7Drainage tubingSection of the 6.1

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭31212134Figure 10 Capillary feed-throughs on the doors1Luer

3.8 Capillary and cable feed-throughs￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭32￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient1234567 88 76 59101112 1211109Fron

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭333.9 Eluent3.9.1 Connecting eluent bottleThe eluent is a

3.9 Eluent￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭34￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient1 234Figure 12 Installing eluent bottle attachment1Eluent asp

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭35 4Installing tubing weighting and aspiration filter1 2345

3.9 Eluent￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭36￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient￭ Fasten the bottle attachment (14-2) on the eluent bottle (1

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭375Eluent6Aspiration filter 6.2821.0907Filter holderFrom ac

3.11 Low pressure gradient￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭38￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient3Tubing flareWith tubing nipple.4Clamping scr

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭39Table 1 Smallest possible eluent percentage depending on

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭IIITable of contents1 Introduction 11.1 Instrument des

3.11 Low pressure gradient￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭40￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientConnecting mixing coil for low pressure gradi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭41Connecting connection tubingsNoteThe connection tubings t

3.12 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭42￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient3.12 High pressure pumpThe intelligent and low

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭435Fastening screwsFor fastening the pump head.6Inlet valve

3.12 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭44￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient123Figure 22 Mixing coil for low pressure gradie

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭45512675345Figure 23 Deaerating the high pressure pump1Syri

3.13 Inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭46￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient 5Aspirating eluent￭ Aspirate with the syringe (23-1)

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭472 32 41Figure 24 Connecting inline filter1Connection capi

3.15 Sample degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭48￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient34523612Figure 25 Pulsation damper – Connection1Con

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭491234Figure 26 Sample degasser1Sample degasser input2Sampl

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭IV￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient3.12 High pressure pump ...

3.16 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭50￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient3.16 Injection valveThe injection valve connects t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭51Replacing the sample loopThe sample loop can be replaced,

3.17 Column thermostat￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭52￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient1Eluent inputCapillary coming from the high press

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭5313211112Figure 29 Column thermostat1Capillary feed-throug

3.17 Column thermostat￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭54￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientNoteThe column input capillary is already threade

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭553.18 Metrohm Suppressor Module (MSM)3.18.1 General info

3.18 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭56￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient13234567821Figure 30 MSM – connecti

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭57Connecting the capillaries of the MSM 1Connecting the elu

3.19 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭58￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient3.19 Peristaltic pump3.19.1 Principle of the per

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭593.19.2 Installing the peristaltic pump6.2744.16016.2744.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭V5.4 Eluent ...

3.19 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭60￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient 3Connecting the pressure sideNoteDepending on the

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭61123Figure 34 Install pump tubing connection without filte

3.19 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭62￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientPump tubing Adapter6.1826.340 (black/black)6.1826.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭633.20 Metrohm CO2 suppressor (MCS)3.20.1 General informa

3.20 Metrohm CO2 suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭64￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient3Aspirating capillaryFor aspirating ai

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭65938 7312 33456Figure 36 Adsorption cartridge holder1PEEK

3.21 Conductivity detector￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭66￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient 2Removing the caps￭ Remove the two locking c

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭67213Figure 37 Conductivity detector front1IC detector 1.85

3.21 Conductivity detector￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭68￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient132Figure 38 Conductivity detector rear1Detec

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭693 21Figure 39 Connection detector – MCS1Detector input ca

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭VI￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient7.5 Housing ...

3.23 Guard column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭70￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientThe mains cable is three-core and provided with a plug

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭71NoteThe guard column may only be installed after the init

3.24 Separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭72￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient3.24 Separation columnThe intelligent separation

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭73Connecting and rinsing the separation column 1Connecting

4.1 Initial start-up￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭74￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient4 Start-upThe chapter Start-up is divided into 2 s

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Start-up850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭75 4Setting the contact pressure of the peristaltic pumpNoteThi

4.2 Conditioning￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭76￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient 2Preparing the instrument￭ Ensure that the column is c

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭775 Operation and maintenance5.1 General inf

5.2 Capillary connections￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭78￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient5.1.3 OperationCautionIn order to avoid distu

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭795.3 DoorCautionThe door is made of PMMA (po

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of figures850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭VIITable of figuresFigure 1 Front 850 Professional IC – A

5.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭80￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient5.4.2 Operation5.4.2.1 Supply bottleThe supply

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭81CautionIn order to spare the pump seals, the

5.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭82￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP GradientCleaning/replacing the zirconium oxide pistonClea

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭83￭ Remove the backup ring from the pump head

5.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭84￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradientparts: a tip for removing the old piston seal and

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭8512Figure 43 Removing the piston seal1Piston

5.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭86￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient12Figure 45 Inserting the piston seal into the pu

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭871234Figure 46 Removing valves1Outlet valve h

5.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭88￭￭￭￭￭￭￭￭850 Professional IC – Anion – MCS – LP Gradient￭ Place the valve with the seal faced downwards a

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – Anion – MCS – LP Gradient￭￭￭￭￭￭￭￭89123567891098710546Figure 48 Components of th