Metrohm 930 Compact IC Flex SeS User Manual download pdf (Page 58)

3.18 Conditioning

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930 Compact IC Flex SeS (2.930.1400)

Installing the output of the separation column

￭ Fasten the column output capillary to the upper end of the sepa-

ration column using a PEEK pressure screw (6.2744.070).

Inserting the separation column

￭ Insert the separation column with chip into the column holder

until you hear it snap in place.

The separation column is now detected by MagIC Net.

3.18 Conditioning

After installation, after each time the instrument is switched on and after

each time the eluent is changed, the system has to be conditioned with

eluent long enough that a stable baseline is attained.

NOTE

The conditioning time can lengthen considerably after changing the

eluent.

Conditioning the system

Preparing the software

CAUTION

Ensure that the configured flow is not higher than the flow permit-

ted for the corresponding column (refer to the column leaflet and

the chip data record).

￭ Start the MagIC Net computer program.

￭ Open the Equilibration tab in MagIC Net: Work-

place ▶ Run ▶ Equilibration.

￭ Select (or create) a suitable method.

Also see: MagIC Net tutorial and online help.

Preparing the instrument

￭ Ensure that the column is inserted correctly in relation to the flow

direction marked on the sticker (arrow has to point in the direc-

tion of flow).

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Metrohm 930 Compact IC Flex SeS User Manual Page 58