Metrohm 850 Professiona IC Cation Prep 1 User Manual

850 Professional ICCation – Prep 1 – 2.850.1030Manual8.850.8043EN

5.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭90￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Problem Cause Remedy￭ Replace separation column (s

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Technical specifications850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭916 Technical specifications6.1 Reference conditionsThe

6.5 Housing￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭92￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 16.5 HousingDimensionsWidth 365 mmHeight 642 mmDepth 380 mmMaterial of

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Technical specifications850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭93Pressure rangePump 0…50.0 MPa (0…500 bar)Pump head 0…35.

6.10 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭94￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 16.10 Injection valveActuator time typ.100 msMax. operatingpre

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Technical specifications850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭95Electrodes Ring-shaped electrodes made from stainless st

6.15 Safety specification￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭96￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Further connec-tions￭ 1 DSUB 15-pin (female)6.15 Safety

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Conformity and warranty850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭977 Conformity and warranty7.1 Declaration of ConformityT

7.2 Quality Management Principles￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭98￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1EN 61010-1 Safety requirements for electrical equ

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Conformity and warranty850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭99Software developmentSoftware development occurs in terms

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭11 Introduction1.1 Instrument descriptionThe instrument 850 Professi

7.3 Warranty (guarantee)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭100￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Faults arising from circumstances that are not the respon

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭1018 AccessoriesCautionSubject to change without notice!8.1 Scope of

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭102￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Qty. Order no. Description1 6.1608.070 Eluent bottle / 2 L /

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭103Qty. Order no. Description1 6.1807.010 Y connector for tubing i.d. 6

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭104￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Qty. Order no. Description1 6.2151.020 Cable USB A - USB B /

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭105Qty. Order no. Description1 6.2621.050 1/4 in. wrenchFor 1/4 in. scr

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭106￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Qty. Order no. Description1 6.2626.000 Front drain nozzleDra

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭107Qty. Order no. Description1 6.2744.020 Luer/UNF couplingFor IC instr

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭108￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Qty. Order no. Description2 6.2744.070 Pressure screw shortS

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭109Qty. Order no. Description1 6.2816.040 Purging needleWith PTFE tubin

1.1 Instrument description￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 11467825391011Figure 1 Front view of the instrument1Conduc

8.2 Optional accessories￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭110￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 18.2 Optional accessories 2.850.1030 850 Professional IC

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭111Order no. Description6.1826.360 Pump tubing LFL (white/white), 3-sto

8.2 Optional accessories￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭112￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Order no. Description6.2057.120 Filtration cell holderHol

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭113Order no. Description6.6059.002 MagIC Net™ Multi – 5 additional lice

8.2 Optional accessories￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭114￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Order no. Description6.9988.503 Validation Documentation

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭115IndexNumbers/Symbols6.2821.090 aspiration filter ... 666.2821.130 filter

Index￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭116￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Fill ... 50Inject ...

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭117Sample degasser ... 93Technical specificationsEluent degasser

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭39Sample degasserSee Chapter 2.13. Use optional.10Separation columnSee

1.2 Intended use￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Column thermostatThe column thermostat controls the temperature of

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭51.3 About the documentationCautionPlease read through this documenta

1.4 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 11.4 Safety instructions1.4.1 General notes on safetyWarnin

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭7Protection against electrostatic chargesWarningElectronic components

1.4 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1More details about the disposal of your old equipment can be

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭92 Installation2.1 About this chapterThe Installation chapter contai

2.3 Flow diagram￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭10￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1 8Install sample path connections.￭ Connect the sample degasser (i

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭11A more detailed description of the functionality of dialysis can be

2.3 Flow diagram￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭12￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1EluentSampleAcceptor solution3111315924561481121710167Figure 2 Flo

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭133High pressure pumpSee Chapter 2.10.4Purge valveSee Chapter 2.10.1.5

2.3 Flow diagram￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭14￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1EluentSample15792456168113141012113Figure 3 Flow diagram with ultr

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭153High pressure pumpSee Chapter 2.10.4Purge valveSee Chapter 2.10.1.5

2.5 Capillary connections in the IC system￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭16￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 12.5 Capillary connections in the IC sys

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭17PEEK capillaries (poly-etheretherketone)PEEK capillaries are tempera

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭18￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 12.6 Rear of the instrument2.6.1 Rollers and handleIn o

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭19Removing rollersProceed as follows to remove the rollers: 1Remove kn

Metrohm AGCH-9101 HerisauSwitzerlandPhone +41 71 353 85 85Fax +41 71 353 89 [email protected] Professional ICCation – Prep 1 – 2.85

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭20￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1211Figure 6 Handle as MPak holder1Knurled screwsFor fast

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭212.6.2 Positioning and connecting the detectorDetector 1Detector 244

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭22￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1NoteUp to two detectors can be positioned and connected.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭23￭ Connect the detector cable to the detector connection socketDetect

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭24￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1123Figure 8 Connection for the leak sensor on the rear o

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭25245613798Figure 9 Drainage tubing1Drainage tubing connectionFor drai

2.7 Capillary and cable feed-throughs￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭26￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 17Drainage tubingSection of the 6.1816.020 sil

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭27212134Figure 10 Capillary feed-throughs on the doors1Luer connectors

2.7 Capillary and cable feed-throughs￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭28￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 11234567 88 76 59101112 1211109FrontRearFigure

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭292.8 Eluent2.8.1 Connecting eluent bottleThe eluent is aspirated ou

TeachwareMetrohm AGCH-9101 [email protected] documentation is protected by copyright. All rights reserved.Although all the information

2.8 Eluent￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭30￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 11 234Figure 12 Installing eluent bottle attachment1Eluent aspiration tub

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭31 4Installing tubing weighting and aspiration filter1 2345Figure 14 I

2.8 Eluent￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭32￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1￭ Fasten the bottle attachment (14-2) on the eluent bottle (16-10).The a

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭335Eluent6Aspiration filter 6.2821.0907Filter holderFrom accessory set

2.10 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭34￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 13Tubing flareWith tubing nipple.4Clamping screw5Eluent aspi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭3510891234562722211212213Figure 18 Capillary connections high pressure

2.10 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭36￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1NoteThe eluent aspiration tubing is already installed in th

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭372.10.2 Deaerating the high pressure pumpThe high pressure pump will

2.10 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭38￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Deaerating the high pressure pumpThe instrument must be con

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭39 4Setting the flow rate￭ Start MagIC Net (if not yet started).￭ Ensu

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭IIITable of contents1 Introduction 11.1 Instrument description ..

2.12 Pulsation damper￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭40￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Installing the inline filterCautionObserve the flow direction

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭41The pulsation damper protects the separation column from damagecause

2.13 Sample degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭42￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 12.13 Sample degasserThe sample degasser removes gas bubbles a

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭43 4Guide the other end of the connection capillary out of the instrum

2.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭44￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 15Tubing cartridges 6.2755.0006Contact pressure lever7Snap-act

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭45 3Connecting the pressure sideNoteDepending on the use of the perist

2.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭46￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1123Figure 27 Install pump tubing connection without filter1Un

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭47Pump tubing Adapter6.1826.340 (black/black)6.1826.360 (white/white)6

2.15 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭48￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 12.15 Injection valveThe injection valve connects the eluent a

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭49Replacing the sample loopThe sample loop can be replaced, depending

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭IV￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 12.14 Peristaltic pump ...

2.16 Column thermostat￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭50￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 11Eluent inputCapillary coming from the high pressurepump.2El

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭5113211112Figure 30 Column thermostat1Capillary feed-throughsFor guidi

2.16 Column thermostat￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭52￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1NoteThe column input capillary is already threaded into the

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭532.17 Conductivity detectorThe conductivity detector continuously me

2.17 Conductivity detector￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭54￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1132Figure 32 Conductivity detector rear1Detector cableWi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭55123Figure 33 Connection detector – separation column1Detector input

2.19 Guard column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭56￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 11Connecting the mains cable￭ Plug the mains cable into the mains

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭57NoteThe guard column may only be installed after the initial start-u

2.20 Separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭58￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 12.20 Separation columnThe intelligent separation column (iC

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭59Connecting and rinsing the separation column 1Connecting the separat

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭V4.10 Peristaltic pump ...

3.1 Initial start-up￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭60￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 13 Start-upThe chapter Start-up is divided into 2 sections:Ini

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Start-up850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭61The instrument is now ready for installing columns.3.2 ConditioningAfte

3.2 Conditioning￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭62￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1 4Conditioning the systemRinse the system with eluent until the re

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭634 Operation and maintenance4.1 General information4.1

4.2 Capillary connections￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭64￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 14.1.3 OperationCautionIn order to avoid disturbing tempe

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭654.3 DoorCautionThe door is made of PMMA (polymethylmet

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭66￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 14.4.2 Operation4.4.2.1 Supply bottleThe supply bottle with

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭67CautionIn order to spare the pump seals, the pump shoul

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭68￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Cleaning/replacing the zirconium oxide pistonClean one pisto

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭69￭ Remove the backup ring from the pump head and lay to

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭VI￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 17.3 Warranty (guarantee) ...

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭70￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1parts: a tip for removing the old piston seal and a sleeve f

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭7112Figure 37 Removing the piston seal1Piston seal2Tool f

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭72￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 112Figure 39 Inserting the piston seal into the pump head 4Re

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭731234Figure 40 Removing valves1Outlet valve holder2Outle

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭74￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1￭ Place the valve with the seal faced downwards above the re

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭75123567891098710546Figure 42 Components of the inlet val

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭76￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1￭ Place the valve housing over the stacked components and ho

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭77Mounting the pump headNoteTo prevent the pump head from

4.6 Inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭78￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Changing the filterThe flow must be stopped before changing the f

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭794.7 Inline sample preparationTo protect the separation

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of figures850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭VIITable of figuresFigure 1 Front view of the instrument ...

4.8 Rinsing the sample path￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭80￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1 2Aspirating the sample and measuring timeAspirate a sa

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭814.9 Sample degasser4.9.1 OperationIf you are working

4.10 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭82￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 14.10.2 Maintenance4.10.2.1 Pump tubingThe pump tubing used

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭83OrdernumberName Material InnerdiameterUse6.1826.360 Pum

4.11 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭84￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 14.11 Injection valve 4.11.1 ProtectionTo prevent contaminati

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭854.13 Separation column4.13.1 Separating efficiencyWhi

4.14 Quality Management and validation with Metrohm￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭86￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 14.13.4 RegenerationNoteThe reg

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Troubleshooting850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭875 Troubleshooting5.1 Problems and their solutionsProblem Cause

5.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭88￭￭￭￭￭￭￭￭850 Professional IC –Cation – Prep 1Problem Cause RemedySample – Blockage in thesample

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Troubleshooting850 Professional IC –Cation – Prep 1￭￭￭￭￭￭￭￭89Problem Cause RemedyPoor reproducibilityof the retentiontimesElue