Metrohm 850 Professional IC AnCat MSM-HC MCS User Manual

850 Professional ICAnCat – MSM-HC – MCS – 2.850.3040Manual8.850.8028EN

Table of figures￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭VIII￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSFigure 45 Components of the inlet valve and outlet valve .

5.7 Inline sample preparation￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭90￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS5.7 Inline sample preparationTo protect the se

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭91 2Aspirating the sample and measuring timeAspirat

5.9 Sample degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭92￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS5.9 Sample degasser5.9.1 OperationIf you are working wi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭93The MSM-HC consists of 3 suppressor units, which

5.11 High Capacity Metrohm Suppressor Module (MSM-HC)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭94￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS 2Regenerating the MSM-

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭95123 45Figure 47 MSM-HC – Components1Union nut2MSM

5.11 High Capacity Metrohm Suppressor Module (MSM-HC)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭96￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS￭ Check whether solutio

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭975.11.3.3 Replacing parts of the MSM-HCIt may be

5.12 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭98￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS￭ Insert the new MSM-HC rotor A (47-3) in the MSM-HC ho

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭99CautionThe 6.1826.xxx pump tubings consist of PVC

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭11 Introduction1.1 Instrument descriptionThe instrument 850 Pr

5.12 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭100￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSOrdernumberName Material InnerdiameterUse6.1826.330 Pu

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭101 3Mounting filter screw￭ Screw the filter screw

5.14 Conductivity detector￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭102￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS5.14 Conductivity detector5.14.1 MaintenanceCau

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭1035.15.2 ProtectionTo protect the separation colu

5.16 Quality Management and validation with Metrohm￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭104￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS5.16 Quality Management

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Troubleshooting850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭1056 Troubleshooting6.1 Problems and their solutionsProblem

6.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭106￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSProblem Cause RemedyEluent – Evaporation of

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Troubleshooting850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭107Problem Cause RemedyMCS – vacuum pumpdefective.Contact the

6.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭108￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSProblem Cause Remedy￭ Replace separation co

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Troubleshooting850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭109Problem Cause RemedyEluent – Gas bubbles in theeluent.Chec

1.1 Instrument description￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSused for the purpose of protecting other sensitive

7.1 Reference conditions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭110￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS7 Technical specifications7.1 Reference condition

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭1117.5 HousingDimensionsWidth 365 mmHeight 642 mmDe

7.8 Sample degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭112￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSPressure rangePump 0…50.0 MPa (0…500 bar)Pump head 0…35.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭1137.10 Column thermostatType Peltier technique the

7.13 Metrohm CO2 suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭114￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS7.13 Metrohm CO2 suppressor (MCS)Material

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭115Cell tempera-ture20…50 °C in increments of 5 °CTe

7.17 Safety specification￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭116￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS7.17 Safety specificationDesign / Test ￭ EN/IEC/U

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Conformity and warranty850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭1178 Conformity and warranty8.1 Declaration of Conf

8.2 Quality Management Principles￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭118￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSEN 61010-1 Safety requirements for electri

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Conformity and warranty850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭119Software developmentSoftware development occurs in

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭3interactions with the column. The Metrohm analytical columns ar

8.3 Warranty (guarantee)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭120￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSFaults arising from circumstances that are not the

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭1219 AccessoriesCautionSubject to change without notice!9.1 Sco

9.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭122￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSQty. Order no. Description2 6.1608.020 Glass bottle /

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭123Qty. Order no. Description1 6.1803.040 PTFE capillary 0.5 mm i

9.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭124￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSQty. Order no. Description2 6.1816.020 Silicone tubing

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭125Qty. Order no. Description1 6.2151.020 Cable USB A - USB B 1.8

9.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭126￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSQty. Order no. Description1 6.2621.030 Hexagon key 4 m

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭127Qty. Order no. Description1 6.2621.100 Hexagon key 3 mmHexagon

9.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭128￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSQty. Order no. Description1 6.2744.014 Pressure screw

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭129Qty. Order no. Description3 6.2744.070 Pressure screw shortSho

1.4 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSInstruction stepCarry out these steps in the sequence

9.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭130￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSQty. Order no. Description2 6.2816.020 Syringe 10 mL w

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭131Qty. Order no. Description1 6.2837.000 CO2 adsorption cartridg

9.2 Optional accessories￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭132￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSOrder no. Description6.2617.040 Tool for piston sea

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭133Order no. Description6.6059.222 MagIC Net™ 2.2 Professional CD

Index￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭134￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSIndexNumbers/Symbols6.2821.090 aspiration filter ... 776.2821.130 f

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭135Protection ... 26, 77Technical specifications ... 1

Index￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭136￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSSeparation column ... 70Rinsing time ...

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭5WarningOnly personnel qualified by Metrohm are authorized to ca

1.4 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS1.4.3 Tubing and capillary connectionsCautionLeaks in

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭72 Overview of the instrument2.1 Front1234234556

2.1 Front￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3High pressure pumpSee Chapter 3.10, Page 37.4Purge valveFor deaerat

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭92.2 Rear12221219201017157654318119121381416Figur

2.2 Rear￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭10￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3Mains switchFor switching the instrument on and off.I = ON0 = OFF4M

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭11CautionWhen connecting an instrument to the MSB

3.1 About this chapter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭12￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3 Installation3.1 About this chapterThe Installation

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭13￭ Place the detectors in the instrument and connect them (seeC

3.2 Initial installation￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭14￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS￭ Connect a part of the 6.1803.040 PTFE capillary wi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭15 8Initial start-up(see Chapter 4.1, page 71)￭ Switch on the PC

3.3 Installation diagram￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭16￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3.3 Installation diagramThe following installation

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭17112345678910111213141516171819202122232425262727272828282930Fi

3.3 Installation diagram￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭18￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3Detector inlet capillary4Conductivity detectorFor t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭1925Rinsing solution outlet capillaryCapillary of the MSM-HC lab

Metrohm AGCH-9101 HerisauSwitzerlandPhone +41 71 353 85 85Fax +41 71 353 89 [email protected] Professional ICAnCat – MSM-HC – MCS –

3.5 Capillary connections in the IC system￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭20￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS412 3Figure 4 Connection of capill

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭21CautionFor the capillary connections between the injection val

3.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭22￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS321145Figure 5 Rollers and handle1Knurled screwsFo

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭23Mounting handle as MPak holderNoteWhen extended, the handle (6

3.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭24￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3.6.2 Positioning and connecting the detectorDete

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭25NoteUp to two detectors can be positioned and connected.Cautio

3.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭26￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS￭ Connect the detector cable to the detector conne

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭27123Figure 8 Connection for the leak sensor on the rear of the

3.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭28￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS245613798Figure 9 Drainage tubing1Drainage tubing

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭297Drainage tubingSection of the 6.1816.020 silicon tubing.Guide

TeachwareMetrohm AGCH-9101 [email protected] documentation is protected by copyright. All rights reserved.Although all the information

3.7 Capillary and cable feed-throughs￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭30￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS212134Figure 10 Capillary feed-throughs

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭311234567 88 76 59101112 1211109FrontRearFigure 11 Capillary fee

3.8 Eluent￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭32￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3.8 Eluent3.8.1 Connecting eluent bottleThe eluent is aspirated

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭331 234Figure 12 Installing eluent bottle attachment1Eluent aspi

3.8 Eluent￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭34￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS 4Installing tubing weighting and aspiration filter1 2345Figure 14

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭35￭ Fasten the bottle attachment (14-2) on the eluent bottle (16

3.9 Eluent degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭36￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS5Eluent6Aspiration filter 6.2821.0907Filter holderFrom ac

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭373Tubing flareWith tubing nipple.4Clamping screw5Eluent aspirat

3.10 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭38￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS10891234562722211212213Figure 18 Capillary connection

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭39NoteThe eluent aspiration tubing is already installed in the n

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭IIITable of contents1 Introduction 11.1 Instrument descript

3.10 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭40￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3.10.2 Deaerating the high pressure pumpThe high pre

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭41Deaerating the high pressure pumpThe instrument must be connec

3.11 Inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭42￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS 4Setting the flow rate￭ Start MagIC Net (if not yet start

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭43Installing the inline filterCautionObserve the flow direction

3.12 Pulsation damper￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭44￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSThe pulsation damper protects the separation column fro

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭453.13 Sample degasserThe sample degasser removes gas bubbles a

3.14 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭46￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS 4Guide the other end of the connection capillary out of

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭473Connection capillaryConnected to connector 4. Carries eluent

3.14 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭48￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS124 53 6124 53 6124354312A BFigure 25 Injection valve –

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭493.15 Column thermostatThe column thermostat controls the temp

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭IV￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3.11 Inline filter ...

3.15 Column thermostat￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭50￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS13211112Figure 26 Column thermostat1Capillary feed-thr

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭51NoteThe column input capillary is already threaded into the ca

3.16 High Capacity Metrohm Suppressor Module (MSM-HC)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭52￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3.16 High Capacity Met

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭5313234567821Figure 27 MSM-HC – Connectors1Union nut2MSM-HC conn

3.16 High Capacity Metrohm Suppressor Module (MSM-HC)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭54￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSConnecting the capillar

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭553.17 Peristaltic pump3.17.1 Principle of the peristaltic pum

3.17 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭56￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3.17.2 Installing the peristaltic pump6.2744.16016.274

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭57 3Connecting the pressure sideNoteDepending on the use of the

3.17 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭58￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS123Figure 31 Install pump tubing connection without fil

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭59Pump tubing Adapter6.1826.340 (black/black)6.1826.360 (white/w

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭V5.5 High pressure pump ...

3.18 Metrohm CO2 suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭60￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3.18 Metrohm CO2 suppressor (MCS)3.18.1 G

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭613Aspirating capillaryFor aspirating air low in CO2 (as a resul

3.18 Metrohm CO2 suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭62￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS938 7312 33456Figure 33 Adsorption cartridg

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭63 2Removing the caps￭ Remove the two locking caps at the tip of

3.19 Conductivity detector￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭64￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS213Figure 34 Conductivity detector front1IC detect

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭65132Figure 35 Conductivity detector rear1Detector cableWith mou

3.20 Connecting the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭66￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS3 21Figure 36 Connection detector – MCS1Detect

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭67The mains cable is three-core and provided with a plug with gr

3.21 Guard column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭68￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSNoteThe guard column may only be installed after the initia

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭693.22 Separation columnThe intelligent separation column (iCol

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭VI￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS7.7 High pressure pump ...

3.22 Separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭70￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSConnecting and rinsing the separation column 1Connecti

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Start-up850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭714 Start-upThe chapter Start-up is divided into 2 sections:Initial

4.2 Conditioning￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭72￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS 4Setting the contact pressure of the peristaltic pumpNoteTh

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Start-up850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭73 2Preparing the instrument￭ Ensure that the column is correctly mo

5.1 General information￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭74￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS5 Operation and maintenance5.1 General information5

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭755.1.3 OperationCautionIn order to avoid disturbi

5.3 Door￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭76￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS5.3 DoorCautionThe door is made of PMMA (polymethylmetacrylate). It

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭775.4.2 Operation5.4.2.1 Supply bottleThe supply

5.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭78￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSCautionIn order to spare the pump seals, the pump shou

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭79Cleaning/replacing the zirconium oxide pistonClea

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of figures850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭VIITable of figuresFigure 1 Front 850 Professional IC – AnCat

5.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭80￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS￭ Remove the backup ring from the pump head and lay to

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭81parts: a tip for removing the old piston seal and

5.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭82￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS12Figure 40 Removing the piston seal1Piston seal2Tool

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭8312Figure 42 Inserting the piston seal into the pu

5.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭84￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS1234Figure 43 Removing valves1Outlet valve holder2Outl

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭85￭ Place the valve with the seal faced downwards a

5.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭86￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCS123567891098710546Figure 45 Components of the inlet va

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭87￭ Place the valve housing over the stacked compon

5.6 Inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭88￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MSM-HC – MCSMounting the pump headNoteTo prevent the pump head from bei

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance850 Professional IC – AnCat – MSM-HC – MCS￭￭￭￭￭￭￭￭89Changing the filterThe flow must be stopped befor