Metrohm 940 Professional IC Vario ONE/SeS/PP/Prep 1 User Manual

940 Professional IC Vario940 Professional IC Vario ONE/SeS/PP/Prep 1Manual8.940.8019EN

5.12 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭90￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510) 2Dismantling the suppressor￭ Unscrew union nut

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭91￭ Insert the rotor (24-3) into the

5.12 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭92￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Replacing parts of the suppressor 1Disconnectin

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭93￭ The rotor's sealing surface

5.13 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭94￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)5.13 Peristaltic pump5.13.1 Notes on op

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭955.13.2.2 Replacing the filterThe f

5.14 Servicing the Metrohm CO2 Suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭96￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)5.14 Servicing

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭97 2Regenerating the adsorber materia

5.17 Separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭98￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)The time upon stopping the watch corresp

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭99representative) or on the Internet

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭11 Introduction1.1 Instrument descriptionThe 940

5.18 Quality Management and qualification with Metrohm￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭100￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)5.18 Q

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Troubleshooting940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭1016 TroubleshootingProblem Cause RemedyMarked

6.1 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭102￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Problem Cause RemedyNote: 6.2821.180 pump tubing connecti

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Troubleshooting940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭103Problem Cause RemedyPeristaltic pump – Pumpt

6.1 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭104￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Problem Cause Remedy￭ Deaerate the high-pressure pump (se

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭1057 Technical specifications7.1 Ref

7.4 Housing￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭106￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)7.4 HousingDimensionsWidth 365 mmHeight 642 mmDep

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭1077.7 Eluent degasserMaterial Fluoro

7.9 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭108￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Gradient capabil-ityIsocratic or gradient

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭1097.12 Metrohm CO2 Suppressor (MCS)M

1.1 Instrument description￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Leak sensorThe leak sensor detects le

7.16 Interfaces￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭110￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)7.16 InterfacesUSBInput 1 USB upstream, type

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭1117.18 Electromagnetic compatibility

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭112￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)8 Warranty (guarantee)Metrohm guarantees that the deliveries

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Warranty (guarantee)940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭113The same warranty periods that are spec

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭114￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)9 AccessoriesUp-to-date information on the scope of delivery

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭115The Partslists webpage will be displayed. 7Selec

Index￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭116￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)IndexAAdsorption cartridgesConnection ...

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭117LLeak ... 70Leak s

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭3Peristaltic pumpThe peristaltic pump is used for

1.3 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)1.3 Safety instructions1.3.1 General n

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭5Protection against electrostatic chargesWARNINGEl

1.4 Symbols and conventions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)More details about the disposal of y

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭72 Overview of the instrument2.1 F

2.1 Front￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)5Suppressor6Column thermostatWith two column holders f

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭92.2 RearFigure 2 Rear1Bottle holde

2.3 Feed-throughs for capillaries and cables￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭10￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)5Vacuum connection

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭11Openings on the doorFigure 3 Feed-

2.3 Feed-throughs for capillaries and cables￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭12￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Openings on the ba

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭13Figure 5 Ducts for capillaries

3.1 Setting up the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭14￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)3 Installation3.1 Setting up th

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭15Also see: PEEK pressure screws video on the Inte

3.2 Capillary connections in the IC system￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭16￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭ The capillary ends

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭173.3 Removing the handleThe instrument is equipp

3.4 Removing transport locking screws￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭18￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)3.4 Removing transport l

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭19Store the transport locking screws in a safe pla

Metrohm AGCH-9100 HerisauSwitzerlandPhone +41 71 353 85 85Fax +41 71 353 89 [email protected] Professional IC Vario940 Professional

3.5 Connecting the drainage tubing and leak sensor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭20￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Connecting t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭21Route the loose end into a waste container.3.5.2

3.7 Connecting the eluent bottle￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭22￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)3.7 Connecting the eluent bot

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭23 2Mounting the tubing adapter and the aspiration

3.7 Connecting the eluent bottle￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭24￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510) 4Mounting the adsorber tubeNO

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭253.8 Connecting the eluent degasserThe high-pres

3.10 Installing an inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭26￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Figure 9 High-pressure pump wi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭27Figure 10 Inline filter1Input capillaryConnected

3.12 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭28￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)3.12 Injection valveThe injection valve c

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭29Figure 12 Exchanging the sample loop1Pressure sc

TeachwareMetrohm AGCH-9100 [email protected] documentation is protected by copyright. All rights reserved.Although all the information

3.13 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭30￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)3.13 SuppressorThe suppressor drive of the 940

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭31CAUTIONThe rotor may be destroyed during start-u

3.13 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭32￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)NOTEThe rotor's sealing surface is approxi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭33￭ Insert the rotor into the adapter so that the

3.13 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭34￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Recommended installationAlternative installatio

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭353.13.2.1 Installing the eluent pathThe eluent p

3.13 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭36￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510) 1Prepare a tubing cartridge of the peristaltic

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭37Connecting the rinsing solution inlet to the per

3.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭38￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Order num-berName Material Inner diame-te

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭39Pump tubing Adapter6.1826.390 (yellow/yellow)6.1

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭IIITable of contents1 Introduction 11.1 Instr

3.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭40￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Installing the pump tubingFor this step y

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭41￭ Install the pump tubing connection with safety

3.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭42￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Input Tighten the aspiration tubing (6.18

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭43Setting the contact pressure correctly 1￭ Fully

3.15 Metrohm CO2 Suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭44￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Figure 14 Peristaltic pump1Co

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭45ymer membrane. At the same time, the vacuum pump

3.15 Metrohm CO2 Suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭46￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510) 3Connection to the detectorC

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭47 1Preparing the adsorption cartridge holder￭ Pus

3.16 Installing the detector￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭48￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)3.16 Installing the detectorThe 9

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭49Connecting the sample degasserCAUTIONIf the samp

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭IV￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)3.13.2 Connecting the suppressor ...

3.18 Connecting the instrument to a computer￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭50￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)3.18 Connecting t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭513.19 Connecting the instrument to the power sup

3.20 Initial start-up￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭52￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)After being switched on, the LED on the f

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭53MagIC Net detects the instrument and all of its

3.21 Connecting and rinsing the guard column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭54￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510) 5Setting the cont

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭55NOTEThe guard column may not be connected until

3.22 Connecting the separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭56￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭ Optional: In case the g

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭57CAUTIONNew separation columns are filled with so

3.22 Connecting the separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭58￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Connecting the separation

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭59 2Installing the input of the separation columnT

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭V5.12.3 Servicing the suppressor ...

3.23 Conditioning￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭60￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)3.23 ConditioningAfter installation, after e

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭61￭ Visually inspect whether all capillaries and t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭62￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)4 OperationThe 940 Professional IC Vario – ONE/SeS/PP/Prep 1

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭635 Operation and maintenance5.1 IC

5.1 IC system￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭64￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)5.1.4 Shutting down and starting back upIf the i

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭655.2 Capillary connectionsAll conne

5.4 Column thermostat – Replacing the capillaries￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭66￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)13211112Figur

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭67Replacing the preheating capillarie

5.6 Servicing the eluent degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭68￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)The composition of the eluent

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭69Connecting the connecting tubing 1￭

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭VI￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)8 Warranty (guarantee) 1129 Accessories 1

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭70￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)5.8 Servicing the high-pr

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭71CAUTIONMaintenance work on the high

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭72￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Cleaning the outlet valve

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭73NOTEThe maximum treatment time in a

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭74￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Spare parts If the inlet v

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭75NOTEThe maximum treatment time in a

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭76￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭ Has the pressure dissipa

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭77Figure 18 High-pressure pump – cros

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭78￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)21Figure 19 Tool for pisto

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭79 3Removing the old piston sealCAUTI

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of figures940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭VIITable of figuresFigure 1 Front ...

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭80￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Optional: Cleaning/replaci

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭81Figure 22 Components of the piston

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭82￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)You can find these parts i

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭83Connecting the input for the high-p

5.9 Servicing the inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭84￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)Removing the filterFigure 23 In

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭85Inserting a new filter 1Inserting a

5.10 Servicing the pulsation absorber￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭86￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)5.10 Servicing the pulsa

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭87CAUTIONThe suppressor has to be reg

5.12 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭88￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)5.12.3.2 Regenerating the suppression rotorIf

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 1 (2.940.1510)￭￭￭￭￭￭￭￭89￭ In the software, use the Step com