Metrohm 844 UV/VIS Compact IC User Manual

844 UV/VIS Compact ICManual8.844.1053

Table of contents 66 Capillary 67 Ferrule (6.2620.010) 68 Ferrule (6.2620.270) 69 Pressure screw (6.2620.000) 70 Steel capillary (6.2620.0

Table of contents Figure 29: UV lamp (deuterium) Figure 30: Lamp connections (at the back of the 844 UV/VIS Compact IC) 9 Lamp cooling housi

4.3 Faults and malfunctions 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 89 4.3 Faults and malfunctions 4.3.1 Error messages If any type

Table of contents Malfunction Cause Rectification Extreme peak broadening, splitting (double peaks) • Dead volume at the co-lumn ends • Che

4.4 Quality Management and validation with Metrohm 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 91 4.4 Quality Management and validation

Table of contents 5 Appendix 5.1 Technical data Unless otherwise specified, the published data comprises typical values for the 844 UV/VIS Com

5.1 Technical data 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 93 5.1.2 Injection valve Actuator switching duration 100 … 150 ms Press

Table of contents 5.1.4 Peristaltic pump Type 2 channel peristaltic pump Pump capacity Rotational speed 20 U/min at 50 Hz 24 U/min at 60 Hz F

5.1 Technical data 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 95 5.1.8 Remote interface Connector Dsub 25 pin (female) Circuit diagr

Table of contents 5.1.10 Analog output Connector Dsub 9 pin (female) Uout = -1V...+1V 5.1.11 Control input Connector Dsub 9 pin (female) Ac

5.1 Technical data 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 97 Immunity to interference Standards met: - EN/IEC 61326 - EN/IEC 610

Table of contents 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 VII 138 Piston guide sleeve (4.709.4380) 139 Piston guide sleeve (4.70

Table of contents 5.2 Standard equipment Subject to changes ! All dimensions are given in mm. 5.2.1 PEEK versions The following PEEK version

5.2 Standard equipment 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 99 Number Order no. Description 2.844.0010 2.844.0020 2.844.0110

Table of contents Number Order no. Description 2.844.0010 2.844.0020 2.844.0110 2.844.0120 Length = 1 m 2 3 - 1 6.1831.110 PEEK capilla

5.2 Standard equipment 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 101 Number Order no. Description 2.844.0010 2.844.0020 2.844.0110

Table of contents Number Order no. Description 2.844.0010 2.844.0020 2.844.0110 2.844.0120 Set of 5 pieces - 1 - 1 6.2744.180 PEEK coup

5.2 Standard equipment 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 103 Number Order no. Description 2.844.0010 2.844.0020 2.844.0110

Table of contents Number Order no. Description 2.844.0030 2.844.0130 1 1 6.1609.000 CO2 Absorber tube incl. 6.2701.020 Stopper For 6.1602.

5.2 Standard equipment 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 105 Number Order no. Description 2.844.0030 2.844.0130 1 1 6.26

Table of contents Number Order no. Description 2.844.0030 2.844.0130 1 1 6.6034.033 Software CD «IC Net 2.3» 1 1 8.102.0033 Software Manu

5.3 Optional accessories 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 107 5.3 Optional accessories 5.3.1 General accessories Order no.

Table of contents Order no. Description 6.2744.070 PEEK compression fitting short Spare part for 6.2824.100 Pump head Set of 5 6.2821.130 F

5.3 Optional accessories 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 109 Order no. Description 6.2824.080 Outlet valve (metal-free)

Table of contents 5.3.4 Separating columns and precolumns This is a selection of standard columns from our comprehensive range of columns. Our

5.3 Optional accessories 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 111 Order no. Description 6.1010.240 Metrosep C2 S-Guard Separa

Table of contents 5.4 Warranty and Conformity 5.4.1 Warranty (guarantee) Metrohm guarantees that the deliveries and services it provides ar

5.4 Warranty and Conformity 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 113 5.4.2 Declaration of Conformity This is to certify the c

Table of contents 5.4.3 Quality Management Principles Metrohm Ltd., CH-9101 Herisau, Switzerland CH-9101 Herisau/Switzerland E-Mail info@m

5.5 Index 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 115 5.5 Index < <Close> 77 <Copy> 77 <Inject> 65 <Optimize

Table of contents Contaminated valves 79 Control input 5 Controls 65 Cotton wool Insert 33 Coupling Attaching pump tubing 36 Figure 36 Ordering

5.5 Index 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 117 K Knurled screw Figure 44, 45 Knurled screws 49 Kolbendichtung Bestellbezeichn

1.1 Instrument description 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 1 1 Introduction 1.1 Instrument description The 844 UV/VIS Com

Table of contents Pressure screw 22 Pressure# drop 90 measurement 94 range 94 rise 90 Program 67 Installation 28 Program instructions 68, 69 Pr

5.5 Index 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 119 Installation 32 Threshold 74 Time program 67 Enable 68 Verify 68 Transport 20

Table of contents The two steel versions 2.844.0030 and 2.844.0130 are used for HPLC applications. There is an addittional componet of stel ver

1.2 Parts and controls 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 3 1.2 Parts and controls In this Section you will find the numbers

Table of contents 1Door to inner compartment 2Feedthrough for eluent, sample, waste, reagent... 3LED "Power" lights up when the inst

1.2 Parts and controls 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 5 1.2.2 Rear panel Figure 2: Rear 844 UV/VIS Compact IC 2 Feedthrou

Table of contents 16USB-B connection 17USB-A connection 18RS232 interface PC connection 19Transport security screws to secure the pump head wh

1.2 Parts and controls 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 7 1.2.3 Interior PEEK versions Figure 3: Interior PEEK versions 27

Table of contents 24Connection capillary Connection pump head – purge valve, fixed mounting 25Connection capillary in pump head, fixed mountin

1.2 Parts and controls 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 9 1.2.4 Interior Steel versions Figure 4: Interior Steel Versions

Table of contents 55Steel fastening screws for steel pump head 54 56Steel connection capillary Steel capillary 6.2620.020 from steel purge valv

1.3 Information on the Instructions for Use 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 11 1.3 Information on the Instructions for Use

Table of contents 1.3.2 Notation and pictograms The following notations and pictograms (symbols) are used in these Instructions for Use: Fill

1.4 Safety notes 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 13 1.4 Safety notes 1.4.1 Electrical safety While electrical safety in the

Table of contents 2 Installation 2.1 Overview 2.1.1 Flow chart The following flow chart provides an overview of all installation work. You will

2.1 Overview 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 15 2.1.2 Connections in the 844 UV/VIS Compact IC The following illustrations

Table of contents Figure 6: Connecting diagram for 844 UV/VIS Compact IC 2.844.0020 with Post column reactor Waste UV/VIS Detector Sample El

2.1 Overview 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 17 Figure 7: Connecting diagram for 844 UV/VIS Compact IC 2.844.0030 8786Fehl

Metrohm AGCH-9101 HerisauSwitzerlandPhone +41 71 353 85 85Fax +41 71 353 89 [email protected] UV/VIS Compact ICManual8.844.1053 03.

Table of contents Figure 8: Connecting diagram for 844 UV/VIS Compact IC 2.844.0110 with Column heating Waste UV/VIS Detector Sample Eluent I

2.1 Overview 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 19 Figure 9: Connecting diagram for 844 UV/VIS Compact IC 2.844.0120 with Pos

Table of contents Figure 10: Connecting diagram for 844 UV/VIS Compact IC 2.844.0130 with Column heating 2.2 Setting up the instrument 2.2.1

2.3 Attaching the accessories 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 21 2.2.3 Location Position the instrument in the laboratory a

Table of contents 64 66 65 6.2744.070 PEEK compression fitting (short). The connection pieces are mounted on the capillaries as follows: PEEK

2.3 Attaching the accessories 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 23 68 69 70 67 1 Mount connectors Slide a pressure screw 69

Table of contents Figure 12: 6.2821.120 Filter unit PEEK 29 Connection capillary 6.1831.010 PEEK capillary, length L = 13 cm 34 Filter unit P

2.4 Mains connection 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 25 74 Steel connection capillary 6.2620.020 from steel purge valve to

Table of contents voltage range can be read normally and the adjacent white arrow points to the white bar imprinted below the fuse holder. Fig

2.5 Connection to PC 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 27 2.4.4 On/off switching of the instrument The 844 UV/VIS Compact IC

TeachwareMetrohm AGCH-9101 [email protected] documentation is protected by copyright. All rights reserved.Although all the information

Table of contents The installed files (incl. system and method files) are generally not write-protected. To prevent these files from being de

2.6 High-pressure pump 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 29 2.6 High-pressure pump In order to avoid damage to the pump it m

Table of contents Figure 15: Connection of the pulsation dampener 29 Connection capillary 6.1831.010 PEEK capillary, length L = 13 cm 32 P

2.6 High-pressure pump 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 31 2.6.3 Connecting the eluent bottle The eluent supply line from t

Table of contents 3 Mount CO2 absorber tube • First place a piece of cotton wool 89 followed by CO2 absorber 88 (e.g. Merck soda lime pellets

2.6 High-pressure pump 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 33 2 Open and connect system • Start the «IC Net» PC program, if

Table of contents 4 Deaerate pump • Make sure that the aspirating tubing 82 for the high-pressure pump has been immersed into the eluent. • C

2.7 Post column reactor 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 35 2 Attach coupling 93 • At the left-hand end of pump tubing 94

Table of contents 94 Pump tubing 6.1826.320 for reagent 95 Stopper (orange-yellow) 96 Tubing cartridge (6.2755.000) 97 Contact pressure lever

2.7 Post column reactor 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 37 Figure 18: Attaching the post column reactor 49 Peristaltic p

Table of contents 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 I Table of contents 1 Introduction ...

Table of contents Figure 19: Post column reactor The post column reactor connections to the column and detector are described in Section 2.8

2.8 Columns 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 39 • Prepare aspirating tubing 92: cut a piece of 6.1803.020 PTFE tubing to the

Table of contents New IC precolumns are normally filled with solution and sealed at both ends. Before the precolumn is installed in the syste

2.8 Columns 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 41 2.8.3 Connecting the separating column (installation without column heating

Table of contents 111/ 112/ 107 44 46/60 113 44 114 47/110114 Figure 21: Column connection without column heating 44 Mounting rail for column

2.8 Columns 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 43 2.8.4 Connecting the separating column (installation with column heating) 2

Table of contents 119Magnetic holder for fastening the column heater in the inner compartment of the 844 UV/VIS Compact IC 120Model plate with

2.8 Columns 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 45 117 Cover of column heating 121 Feedthrough for column inlet capillary 47/11

Table of contents 3 Insert separating column • Push column 113 into the holding clips and fasten column inlet capillary 47/110 to the inlet

2.8 Columns 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 47 2.8.4.3 Installing the column heating Figure 24: Installing the column hea

Table of contents 2.8.1 Precolumns ... 39 2

Table of contents 2.9 Detector The 844 UV/VIS Compact IC is equipped with UV/VIS detector. The eluent (and the sample) are led through a measur

2.9 Detector 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 49 2 Attach flow-through cell • Attach flow-through cell 42/62 to the optica

Table of contents pressure screw (6.2620.000) 69. 2 Connect flow-through cell outlet • with 2.844.0010, 2.844.0030, 2.844.0110 and 2.844.01

2.10 Putting into operation 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 51 • Select the Connect to workplace item from the Control men

Table of contents starts up. If column heating has been selected on the tab Configuration (see Section 3.3.1) then the column heating will also

2.10 Putting into operation 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 53 • Select the Connect to workplace item from the Control menu

Table of contents 5 Check for leaks • Check all the capillaries and their connections from the high-pressure pump to the detector block for

2.11 Connection of external devices 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 55 2.11 Connection of external devices 2.11.1 Connect

Table of contents 5 Setting up the 844/838 system • Select File / Open / System in the main window of the «IC Net » software. Open the syste

2.11 Connection of external devices 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 57 Net» Software Instructions for Use, Section 6.26.3.

Table of contents 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 III 5.1.6 Gas Sensor...

Table of contents the openings of the 844 UV/VIS Compact IC and use a 6.2744.010 PEEK Compression fitting to screw it onto injection valve 46/6

3.1 «IC Net» - Terms 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 59 3 Operation This Section describes only the most important points

Table of contents 3.2.2 Defining measuring channels With the 844 UV/VIS Compact IC up to four measuring channels can be recorded simultaneo

3.2 Measuring operation 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 61 Assign data sources A data source is assigned to each of these d

Table of contents 3.2.4 Instrument icon When you click with the right mouse button on the instrument icon, you will see the following menu: Op

3.3 Settings 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 63 (on the tab Configuration) will be set on the 844 UV/VIS Compact IC. Depend

Table of contents Pump pressure, MPa Displays the currently measured pressure of the high-pressure pump (units depend on the settings made un

3.3 Settings 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 65 Pressure max, MPa Value for maximum switch-off pressure for high-pressure

Table of contents VIS lamp VIS lamp <On> Turn VIS lamp on. <Off> Turn VIS lamp off. <Zero> Records a new zero spectrum. Th

3.3 Settings 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 67 The Program tab contains the two following subtabs: Program Main time pro

Table of contents List of figures Figure 1: Front 844 UV/VIS Compact IC... 3 F

Table of contents List of program instructions The following program instructions can be added to the time program on the Program subtab: Remot

3.3 Settings 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 69 Name (1st column) User-definable name of the remote command (e.g. Start_83

Table of contents Peristaltic pump If this option is switched off the peristaltic pump will not be switched on at Startup hardware or at the s

3.3 Settings 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 71 Event Events for automatic remote signal output: Inject Switching of the

Table of contents Shutdown hardware after.. Event for alarm stop: Pump stop Pump stopped because pressure limits are exceeded. Hardware erro

3.3 Settings 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 73 Flow correction Factor for correction of the difference bet-ween displayed

Table of contents T int, ms Integration time. Entry range: 10 ... 254 ms T out, s Measuring time per data point. Entry range: 0.05 ... 2.00 s

3.3 Settings 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 75 A selection can be made from three different spectra, the absorption spect

Table of contents refaktIntrefzeroIntaktIntzeroIntAbsλλλλloglog11−= where: refzeroIntλ The intensity measured when recording the zero spectrum

4.1 Practical notes on ion chromatography 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 77 4 Notes - Maintenance - Faults 4.1 Practical

Table of contents 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 V List of numbered parts and controls 1 Door to inner compartment 2 F

Table of contents Storage Always store the separating columns sealed when not in use and filled in accordance with the manufacturer’s specifica

4.1 Practical notes on ion chromatography 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 79 Fresh eluents should always be microfiltered (

Table of contents 4.2 Maintenance and servicing 4.2.1 General information Care The 844 UV/VIS Compact IC requires proper care and attention. Ex

4.2 Maintenance and servicing 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 81 If you wish to replace an IC separating column by a column

Table of contents 4 Rinse column • Place beaker beneath the column outlet. • Set flow rate for new separating column. • Switch on high-pressu

4.2 Maintenance and servicing 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 83 4 Replacement of piston seal 140 • To remove damaged pi

Table of contents Figure 27: Replacement of the piston seal 140 27/54 Pump head 6.2824.040/6.2824.100 133 Screw (3.709.1100) for piston cartr

4.2 Maintenance and servicing 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 85 through the upper opening in the valve housing. The indivi

Table of contents Figure 28: Components of inlet valve 141 and outlet valve 142 4.2.5 Replacing the pump tubing The pump tubings used by th

4.2 Maintenance and servicing 844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051 87 2 Insert new pump tubing • Insert the new pump tubing